Human Fibroblast growth factor receptor 3(FGFR3) ELISA kit

Human Fibroblast growth factor receptor 3(FGFR3) ELISA kit

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Mfr.No.

CSB-EL008646HU

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fibroblast growth factor receptor 3, ACH, CD333, CEK2, HSFGFR3EX, JTK4, OTTHUMP00000149958, OTTHUMP00000149959, achondroplasia, thanatophoric dwarfism, hydroxyaryl-protein kinase, tyrosine kinase JTK4

Recovery

The recovery of human MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	96	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	93-105
1:2	Average %	92
	Range %	85-99
1:4	Average %	103
	Range %	95-108
1:8	Average %	96
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.244	2.404	2.324	2.122
10	1.556	1.587	1.572	1.370
5	1.062	1.081	1.072	0.870
2.5	0.709	0.736	0.723	0.521
1.25	0.476	0.499	0.488	0.286
0.625	0.374	0.397	0.386	0.184
0.312	0.330	0.336	0.333	0.131
0	0.201	0.203	0.202	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P22607

Applications	ELISA
Research Area	FGFR3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502389	CSB-EL008646HU	96T	$695	Add to Cart
BHE10502389	CSB-EL008646HU	5x96T	$3336	Add to Cart
BHE10502389	CSB-EL008646HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.