Human fibroblast growth factor 9 (glia-activating factor) (FGF9) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04550h

Alternative Names

fibroblast growth factor 9 (glia-activating factor), GAF, HBFG-9, MGC119914, MGC119915, SYNS3, fibroblast growth factor 9

Reactivity

Human

Detection Range

6.25 pg/mL-400 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human ADAMTS-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	88
	Range %	80-92
1:2000	Average %	98
	Range %	91-105
1:4000	Average %	101
	Range %	92-110
1:8000	Average %	94
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

1.56 pg/mL

Limit Of Detection

450 nm

UniProt ID	P31371
Antigen	fibroblast growth factor 9 (glia-activating factor)

Applications

ELISA

Research Area

FGF9

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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