Human Fibroblast Growth Factor 2

Mfr.No.

E0055Hu

Sensitivity

2.49ng/L

Detection Range

5-1500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Fibroblast Growth Factor 2 (also known as FGF2) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

Basic fibroblast growth factor;bFGF;FGF 2;FGF2;FGF-2;Fibroblast growth factor 2;HBGF-2;Heparin-binding growth factor 2

Molecular Weight

30770

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E0055Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	229.7	11.89	5.2
Intra-Assay	2	18	566.3	16.48	2.9
Intra-Assay	3	18	121.2	7.93	6.5

Background

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed: 8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed: 28302677). Binds to integrin ITGAV:ITGB3 (PubMed: 28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed: 8663044, PubMed: 28302677). Functions as a potent mitogen in vitro (PubMed: 1721615, PubMed: 3964259, PubMed: 3732516). Can induce angiogenesis (PubMed: 23469107, PubMed: 28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed: 29501879).

Protocol

These standard curves of E0055Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.373	2.344	2.297
	2.315
400ng/L	1.203	1.234	1.187
	1.264
200ng/L	0.636	0.634	0.586
	0.632
100ng/L	0.509	0.457	0.41
	0.404
50ng/L	0.199	0.213	0.165
	0.226
0ng/L	0.051	0.047	0
	0.043

Gene ID	2247
UniProt ID	P09038

Research Area	Cardiovascular; Signal Transduction; Neuroscience; Cancer

Storage	2-8°C

Reference	Effect of Vitamin E on Serum Levels of Vascular Endothelial Growth Factor and Angiopoietin-1 in Women with Polycystic Ovary Syndrome: A Pilot Randomized, Placebo-Controlled Trial Joual: Int J Fertil Steril. 2021 Jan;15(1):44-50. Effect of Vitamin E on Serum Levels of Vascular Endothelial Growth Factor and Angiopoietin-1 in Women with Polycystic Ovary Syndrome: A Pilot Randomized, Placebo-Controlled Trial Joual: Int J Fertil Steril. 2021 Jan-Mar; 15(1): 4450.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101790	E0055Hu	96T	$458		Add to Cart
BHE12101790	E0055Hu	48T	$320		Add to Cart

Human Fibroblast Growth Factor 2 - FGF2 ELISA Kit

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