Human Fibroblast growth factor 19(FGF19) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL008624HU

Alternative Names

fibroblast growth factor 19

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human MYH11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-101
EDTA plasma (n=4)	88	82-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MYH11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-99
1:2	Average %	102
	Range %	97-105
1:4	Average %	98
	Range %	92-101
1:8	Average %	95
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	O95750
Antigen	Fibroblast Growth Factor 19

Applications

ELISA

Research Area

FGF19

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10502377	CSB-EL008624HU	96T	$695	Add to Cart
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