Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit

Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit

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Mfr.No.

CSB-E14965h

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fibroblast growth factor 10, keratinocyte growth factor 2, produced by fibroblasts of urinary bladder lamina propria, fibroblast growth factor 10, keratinocyte growth factor 2, produced by fibroblasts of urinary bladder lamina propria

Recovery

The recovery of human MyD88 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-90
EDTA plasma (n=4)	92	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MyD88 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	100
	Range %	97-104
1:4	Average %	99
	Range %	96-102
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.488	2.520	2.504	2.331
10	2.045	2.068	2.057	1.884
5	1.381	1.352	1.367	1.194
2.5	0.746	0.788	0.767	0.594
1.25	0.465	0.468	0.467	0.294
0.625	0.352	0.368	0.360	0.187
0.312	0.267	0.251	0.259	0.086
0	0.172	0.174	0.173

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O15520

Applications	ELISA
Research Area	FGF10

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502370	CSB-E14965h	96T	$695	Add to Cart
BHE10502370	CSB-E14965h	5x96T	$3336	Add to Cart
BHE10502370	CSB-E14965h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.