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Home / ELISA Kit

Human Fibrinogen beta chain (FGB) ELISA kit

SKU : BHE10502369

This Human EGFL7 ELISA Kit was designed for the quantitative measurement of Human EGFL7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.47 ug/mL-30 ug/mL and the sensitivity is 0.12 ug/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E09611h

Alternative Names

fibrinogen beta chain, MGC104327, MGC120405, fibrinogen, B beta polypeptide, fibrinogen, beta chain

Reactivity

Human

Sample Type

Serum, Plasma, Tissue Homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human EGFL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		92		85-98
EDTA plasma (n=4)		94		91-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human EGFL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		95
		Range %		90-102
1:2		Average %		95
		Range %		83-101
1:4		Average %		96
		Range %		90-106
1:8		Average %		94
		Range %		82-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average	Corrected
30	2.753	2.618	2.686	2.572
15	2.421	2.404	2.413	2.299
7.5	1.894	1.877	1.886	1.772
3.75	1.186	1.197	1.192	1.078
1.87	0.610	0.634	0.622	0.508
0.94	0.411	0.407	0.409	0.295
0.47	0.265	0.271	0.268	0.154
0	0.112	0.115	0.114	?

Species

Homo sapiens

Limit Of Detection

450 nm

UniProt ID	P02675
Antigen	Fibrinogen beta chain

Applications

ELISA

Research Area

FGB

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10502369	CSB-E09611h	96T	$790		Add to Cart
BHE10502369	CSB-E09611h	5x96T	$3792		Add to Cart
BHE10502369	CSB-E09611h	10x96T	$7505		Add to Cart

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