Human Fibrinogen alpha chain (FGA) ELISA kit

Human Fibrinogen alpha chain (FGA) ELISA kit

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Mfr.No.

CSB-EL008607HU

Sensitivity

0.47 ug/mL

Detection Range

0.94 ug/mL-60 ug/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Fibrinogen alpha chain (FGA), Fib2, MGC119422, MGC119423, MGC119425, fibrinogen, A alpha polypeptide, fibrinogen, alpha chain, isoform alpha preproprotein, fibrinogen, alpha polypeptide

Recovery

The recovery of chicken GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	88-96
EDTA plasma (n=4)	94	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	99
	Range %	92-105
1:4	Average %	85
	Range %	80-89
1:8	Average %	90
	Range %	83-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
10000	0.154	0.145	0.150
5000	0.261	0.244	0.253
2500	0.538	0.534	0.536
1250	1.113	1.139	1.126
625	2.356	2.278	2.317

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P02671

Applications	ELISA
Research Area	FGA

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502363	CSB-EL008607HU	96T	$695	Add to Cart
BHE10502363	CSB-EL008607HU	5x96T	$3336	Add to Cart
BHE10502363	CSB-EL008607HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.