Human FGF21 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0994

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.017	0.063	0.103	0.195	0.385	0.712	1.422	2.24

Description

Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FGF21 in cell culture supeatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Alternative Names

Fibroblast growth factor 21;FGF-21;FGF21;UNQ3115/PRO10196;

Molecular Weight

22300 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H29-S209

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human FGF21

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Tissue homogenates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	256	645	1478	247	649	1426
Standard deviation	11.52	21.93	69.47	24.45	48.03	89.84
CV (%)	4.5%	3.4%	4.7%	9.9%	7.4%	6.3%

Protein Function

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 [removed]but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. .

Background

Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	26291
Gene Name	FGF21
UniProt ID	Q9NSA1
Protein Name	Fibroblast growth factor 21
Sequence	Belongs to the heparin-binding growth factors family.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cardiovascular,Growth Factors,Growth Factors/Hormones,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000021	EZ0994	5 plates/kit	$500		Add to Cart

Human FGF21 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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