Matrices listed below were spiked with certain level of FGF17 and the recovery rates were calculated by comparing the measured value to the expected amount of FGF17 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-104
98
EDTA plasma(n=5)
85-100
92
heparin plasma(n=5)
91-101
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF17 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-99%
89-103%
90-103%
EDTA plasma(n=5)
85-101%
89-101%
84-99%
heparin plasma(n=5)
85-99%
80-95%
88-97%
Sensitivity
< 0.938pg/ml
Notes
For Research Use Only
UniProt ID
O60258
Applications
ELISA
Applications Notes
FGF17 ELISA Kit allows for the in vitro quantitative determination of FGF17 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human FGF17 (Fibroblast growth factor 17) ELISA Kit