Human fetal Fibronectin(fFN)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E17582h

Sensitivity

6.25 ng/mL

Reactivity

Human

Sample Type

Serum, Cell culture supernatants, Urine, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fetal Fibronectin(fFN)

Recovery

The recovery of human MYCN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-101
EDTA plasma (n=4)	86	82-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MYCN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	99-108
1:2	Average %	97
	Range %	92-102
1:4	Average %	95
	Range %	90-99
1:8	Average %	94
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Antigen	fetal Fibronectin(fFN)

Applications	ELISA
Research Area	fFN

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Endothelial hemostatic predictors of cardiovascular risk in patients with vibration disease in combination with the arterial hypertension Shpagina, L. A.et al,Journal of Siberian Medical Sciences,/ Multimode smartphone biosensing: the transmission, reflection, and intensity spectral (TRI)-analyzer Long KD.et al,Lab Chip. ,Jul 28. Integrated spectroscopic analysis system with low vertical height for measuring liquid or solid assays YuhangWan.et al,Sensors and Actuators B: Chemical,Aug 20

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