Human ferritin,FE ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E05187h

Sensitivity

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Detection Range

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Reactivity

Human

Assay Duration

1-5h

Sample Volume

50-100ul

Alternative Names

ferritin,FE

Recovery

The recovery of mouse C3b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-97
EDTA plasma (n=4)	89	86-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C3b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-91
1:2	Average %	94
	Range %	89-98
1:4	Average %	103
	Range %	99-105
1:8	Average %	99
	Range %	95-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Cell Lysates, Serum, Tissue Homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	FE

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Hepcidin expression and iron parameters change in Type 2 diabetic patients Jiang F et al ,Diabetes Research and Clinical Practice,2011 Jul;93(1):43-8 The Association of Serum Vascular Endothelial Growth Factor and Ferritin in Diabetic Microvascular Disease Guo L et al,Diabetes Technol Ther,2013 Nov 26. A composite mouse model of aplastic anemia complicated with iron overload Dijiong Wu.et al,Experimental and Therapeutic Medicine,Nov 16 Eltrombopag: a powerful chelator of cellular or extracellular iron(III) alone or combined with a second chelator Evangelia Vlachodimitropoulou.et al,Blood.,Sep 1 Comparison of the effects of deferasirox, deferoxamine, and combination of deferasirox and deferoxamine on an aplastic anemia mouse model complicated with iron overload Dijiong Wu.et al,Drug Des Devel Ther.,May 3

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SKU	Supplier No.	Size	Your price	Quantity
BHE10502352	CSB-E05187h	96T	$350		Add to Cart