Human Fermitin family homolog 3(FERMT3) ELISA kit

Human Fermitin family homolog 3(FERMT3) ELISA kit

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Mfr.No.

CSB-EL008595HU

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fermitin family homolog 3 (Drosophila), KIND3, MGC10966, MIG-2, MIG2B, UNC112C, URP2, URP2SF, UNC-112 related protein 2, fermitin family homolog 3, kindlin 3, kindlin-3

Recovery

The recovery of human c-myc spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-96
EDTA plasma (n=4)	92	87-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human c-myc in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-99
1:2	Average %	95
	Range %	91-101
1:4	Average %	92
	Range %	88-96
1:8	Average %	90
	Range %	86-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.404	2.453	2.429	2.341
10	1.587	1.575	1.581	1.493
5	1.084	1.066	1.075	0.987
2.5	0.658	0.699	0.679	0.591
1.25	0.456	0.487	0.472	0.384
0.625	0.251	0.245	0.248	0.160
0.312	0.161	0.169	0.165	0.077
0	0.087	0.089	0.088	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q86UX7

Applications	ELISA
Research Area	FERMT3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502351	CSB-EL008595HU	96T	$695	Add to Cart
BHE10502351	CSB-EL008595HU	5x96T	$3336	Add to Cart
BHE10502351	CSB-EL008595HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.