Matrices listed below were spiked with certain level of FDFT1 and the recovery rates were calculated by comparing the measured value to the expected amount of FDFT1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
80-95
85
EDTA plasma(n=5)
85-98
91
heparin plasma(n=5)
82-100
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FDFT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
94-103%
85-100%
84-103%
EDTA plasma(n=5)
81-100%
82-100%
83-98%
heparin plasma(n=5)
84-99%
82-93%
89-99%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P37268
Applications
ELISA
Applications Notes
FDFT1 ELISA Kit allows for the in vitro quantitative determination of FDFT1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human FDFT1(Squalene synthase) ELISA Kit