Human Fc gamma RIIB/C (CD32b/c)(FCGR2B/C) ELISA Kit PicoKine®

Mfr.No.

EK1312

Sensitivity

<10 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	46.9	93.8	187.5	375	750	1500	3000
O.D.	0.007	0.124	0.199	0.255	0.541	0.813	1.408	2.266

Description

Human Fc gamma RIIB/C (CD32b/c) (FCGR2B/C) ELISA Kit PicoKine® (96 Tests). Quantitate Human FCGR2B/C in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Low affinity immunoglobulin gamma Fc region receptor II-c;IgG Fc receptor II-c;CDw32;Fc-gamma RII-c;Fc-gamma-RIIc;FcRII-c;CD32;FCGR2C;CD32, FCG2, IGFR2;FCGR2B

Molecular Weight

35578 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A46-P217

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human FCGR2B/C

Subcellular Localization

Isoform IIC4: Cytoplasm .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	122	404	1372	133	365	1478
Standard deviation	8.05	19.79	97.41	9.7	22.26	130.06
CV (%)	6.6%	4.9%	7.1%	7.3%	6.1%	8.8%

Protein Function

Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells.

Background

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	122	125	105	106	114	9.06	7.9%
Sample 2	404	361	357	357	369	19.84	5.3%
Sample 3	1372	1213	1353	1369	1326	66.06	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2213, 9103
Gene Name	FCGR2B
UniProt ID	P31994, P31995
Protein Name	Low affinity immunoglobulin gamma Fc region receptor II-b/c
Tissue Specificity	Isoform IIC1 is detected in monocytes, macrophages, polymorphonuclear cells and natural killer cells.
Sequence	Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Applications	ELISA
Research Area	Hematopoietic Progenitors,Immunoglobulins,Immunology,Lymphoid,Myeloid,Receptors,Stem Cells,T Lymphocytic Lineage

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000902	EK1312	96 wells/kit	$750		Add to Cart

Human Fc gamma RIIB/C (CD32b/c)(FCGR2B/C) ELISA Kit PicoKine®

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