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Home / ELISA Kit

Human Fatty acid transport protein 5 (FATP5) ELISA Kit

SKU : BHE13702825

Very long chain fatty acid synthetase (VLACS, or SLC27A2), an enzyme whose function is presumed missing in ALD, is highly expressed in liver and kidney, but is expressed at much lower levels in brain. However, brain contains high amounts of VLCFA and is the organ most affected in ALD. In an attempt to isolate a VLACS isoform with a different tissue distribution, Berger et al. (1998) used degenerate PCR to clone a mouse cDNA and a partial human cDNA encoding a VLACS-related protein, termed VLACSR. VLACSR is 43% identical to mouse VLACS and 37% identical to mouse FATP. Northern blot analysis of mouse tissues revealed that a 2.6-kb VLACSR mRNA was highly abundant in liver. Smaller VLACSR transcripts were present at low levels in brain, lung, testis, spleen, and skeletal muscle.

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Mfr.No.	KTE60609
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Fatty acid transport protein 5 (FATP5) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC27A5.
Alternative Names	SLC27A5; ACSB; ACSVL6; FACVL3; FATP5; FLJ22987; VLACSR; VLCS-H2; VLCSH2; fatty-acid-Coenzyme A ligase; very long-chain 3; very long-chain acyl-CoA synthetase homolog 2; very long-chain acyl-CoA synthetase-rela
Detection Method	Colorimetric
Kit Components	Human Fatty acid transport protein 5 microplate Human Fatty acid transport protein 5 standard Human Fatty acid transport protein 5 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Fatty acid transport protein 5 (FATP5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC27A5. No significant cross-reactivity or interference between Human SLC27A5 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Very long chain fatty acid synthetase (VLACS, or SLC27A2), an enzyme whose function is presumed missing in ALD, is highly expressed in liver and kidney, but is expressed at much lower levels in brain. However, brain contains high amounts of VLCFA and is the organ most affected in ALD. In an attempt to isolate a VLACS isoform with a different tissue distribution, Berger et al. (1998) used degenerate PCR to clone a mouse cDNA and a partial human cDNA encoding a VLACS-related protein, termed VLACSR. VLACSR is 43% identical to mouse VLACS and 37% identical to mouse FATP. Northern blot analysis of mouse tissues revealed that a 2.6-kb VLACSR mRNA was highly abundant in liver. Smaller VLACSR transcripts were present at low levels in brain, lung, testis, spleen, and skeletal muscle.
Notes	This Human Fatty acid transport protein 5 (FATP5) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC27A5 in samples. An antibody specific for SLC27A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC27A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC27A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC27A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10998

Applications	ELISA
Applications Notes	This Human Fatty acid transport protein 5 (FATP5) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC27A5 in samples. An antibody specific for SLC27A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC27A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC27A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC27A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702825	KTE60609	48 T	$359		Add to Cart
BHE13702825	KTE60609	96 T	$619		Add to Cart
BHE13702825	KTE60609	96 Tx5	$2809		Add to Cart
BHE13702825	KTE60609	96 Tx50	$26209		Add to Cart

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