Human Fatty Acid Synthase

Mfr.No.

E3589Hu

Description

This sandwich kit is for the accurate quantitative detection of human Fatty Acid Synthase (also known as FAS) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

(Acyl-carrier-protein) S-malonyltransferase;3-hydroxyacyl-(acyl-carrier-protein) dehydratase;3-oxoacyl-(acyl-carrier-protein) reductase;3-oxoacyl-(acyl-carrier-protein) Synthase;Acyl-(acyl-carrier-protein) hydrolase;Enoyl-(acyl-carrier-protein) reductase;FASN;Fatty acid Synthase;Type I fatty acid Synthase (Includes: (Acyl-carrier-protein) S-acetyltransferase;

Reactivity

Human

Detection Range

0.5-100ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

273427

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.21ng/ml

Background

Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ((ACP)) domain.

Gene ID	2194
UniProt ID	P49327
Antigen	FASN

Research Area

Metabolism

Protocol

These standard curves of E3589Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	2.003	2.028	1.977
	2.054
32ng/ml	1.039	1.038	0.986
	1.036
16ng/ml	0.429	0.446	0.394
	0.463
8ng/ml	0.159	0.173	0.122
	0.188
4ng/ml	0.106	0.129	0.077
	0.152
0ng/ml	0.045	0.052	0
	0.058

Precision

We measured random samples of E3589Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	36.1	1.61	4.5
Intra-Assay	2	18	53.3	1.24	2.3
Intra-Assay	3	18	20.6	0.95	4.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105067	E3589Hu	48T	$320		Add to Cart
BHE12105067	E3589Hu	96T	$458		Add to Cart

Human Fatty Acid Synthase - FAS ELISA Kit

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