Human Fatty acid-binding protein 12(FABP12) ELISA kit

Human Fatty acid-binding protein 12(FABP12) ELISA kit

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Mfr.No.

CSB-EL007941HU

Alternative Names

fatty acid binding protein 12, Probable fatty acid-binding protein ENSP00000353650, fatty acid binding protein ORF, intracellular fatty acid-binding protein FABP12, fatty acid binding protein 12, Probable fatty acid-binding protein ENSP00000353650, fatty acid binding protein ORF, intracellular fatty acid-binding protein FABP12

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat MT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	93	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-89
1:2	Average %	103
	Range %	99-110
1:4	Average %	97
	Range %	92-101
1:8	Average %	90
	Range %	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.937	2.832	2.885	2.718
5	2.048	1.919	1.984	1.817
2.5	1.221	1.274	1.248	1.081
1.25	0.793	0.832	0.813	0.646
0.625	0.518	0.541	0.530	0.363
0.312	0.407	0.399	0.403	0.236
0.156	0.239	0.221	0.230	0.063
0	0.172	0.161	0.167	?

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	A6NFH5
Antigen	fatty acid binding protein 12

Applications

ELISA

Research Area

FABP12

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502342	CSB-EL007941HU	96T	$695	Add to Cart
BHE10502342	CSB-EL007941HU	5x96T	$3336	Add to Cart
BHE10502342	CSB-EL007941HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.