Human Fad-linked Sulfhydryloxidase Alr

Mfr.No.

E4735Hu

Sensitivity

5.54ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Augmenter of liver regeneration;FAD-linked sulfhydryl oxidase ALR;GFER;Hepatopoietin;hERV1

Molecular Weight

23449

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4735Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	713.3	23.54	3.3
Intra-Assay	2	18	198.3	12.3	6.2
Intra-Assay	3	18	380.1	16.1	4.2

Background

(Isoform 1): FAD-dependent sulfhydryl oxidase that regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. (Isoform 2): May act as an autocrine hepatotrophic growth factor promoting liver regeneration.

Protocol

These standard curves of E4735Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.111	2.235	2.178
	2.358
600ng/L	1.217	1.301	1.244
	1.384
300ng/L	0.741	0.752	0.696
	0.764
150ng/L	0.401	0.425	0.368
	0.449
75ng/L	0.271	0.283	0.227
	0.295
0ng/L	0.063	0.056	0
	0.049

Gene ID	2671
UniProt ID	P55789

Research Area	Signal Transduction; Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106024	E4735Hu	48T	$320		Add to Cart
BHE12106024	E4735Hu	96T	$458		Add to Cart

Human Fad-linked Sulfhydryloxidase Alr - GFER ELISA Kit

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