Human factor B,BF ELISA Kit

Human factor B,BF ELISA Kit

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Mfr.No.

CSB-E09447h

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Human

Sample Type

Serum, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

complement factor B, DADB-122G4.5, AHUS4, BF, BFD, CFAB, FB, FBI12, GBG, H2-Bf, PBF2, B-factor, properdin, C3 proaccelerator, C3 proactivator, C3/C5 convertase, OTTHUMP00000174690, glycine-rich beta-glycoprotein

Recovery

The recovery of Human FADD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-94
EDTA plasma (n=4)	98	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Human FADD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	97
	Range %	92-103
1:4	Average %	94
	Range %	89-98
1:8	Average %	98
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.703	2.822	2.763	2.659
4000	2.328	2.208	2.268	2.164
2000	1.615	1.534	1.575	1.471
1000	0.884	0.914	0.899	0.795
500	0.433	0.468	0.451	0.347
250	0.281	0.294	0.288	0.184
125	0.182	0.186	0.184	0.080
0	0.104	0.103	0.104

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P00751
Antigen	Complement factor B

Applications	ELISA
Research Area	CFB

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10502333	CSB-E09447h	5x96T	$3792	Add to Cart
BHE10502333	CSB-E09447h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.