Human FABP4 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ1459

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.044	0.153	0.19	0.309	0.497	1.002	1.482	2.157

Description

Human FABP4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FABP4 in cell culture supeatants, serum, plasma (heparin, EDTA) and cell lysates.

Alternative Names

Fatty acid-binding protein, adipocyte; Adipocyte lipid-binding protein; ALBP; Adipocyte-type fatty acid-binding protein; A-FABP; AFABP; Fatty acid-binding protein 4; FABP4;

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-A132

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human FABP4

Subcellular Localization

Nucleus, Cytoplasm

Sample Type

Cell culture supeatants, Serum, Plasma, Cell lysates

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	237	1431	6434	247	1542	6951
Standard deviation	10.9	97.3	386.04	14.82	114.1	493.52
CV (%)	4.6%	6.8%	6%	6%	7.4%	7.1%

Protein Function

Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.

Background

FABP4, also known as aP2 (adipocyte Protein 2), encodes the fatty acid binding protein found in adipocytes. The FABP4 gene is mapped to 8q21. FABP4 functions as a cytosolic lipid chaperone in macrophages and is involved in regulating macrophage endoplasmic reticulum (ER) stress. Aditionally, FABP4 protein is expressed in HBEs and is strongly upregulated by both IL4 and IL13 and downregulated by IFN-gamma, suggesting a role in allergic airway inflammation.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	2167
Gene Name	FABP4
UniProt ID	P15090

Applications	ELISA
Research Area	Cancer,Cancer Metabolism,Cardiovascular,Fatty Acid Oxidation,Fatty Acids,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Mesenchymal Stem Cells,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Pathways and Processes,Redox Metabolism,Signal Transduction,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000085	EZ1459	5 plates/kit	$500		Add to Cart

Human FABP4 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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