Matrices listed below were spiked with certain level of F8 and the recovery rates were calculated by comparing the measured value to the expected amount of F8 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
95
EDTA plasma(n=5)
88-105
97
heparin plasma(n=5)
85-102
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.