Matrices listed below were spiked with certain level of f-Hb and the recovery rates were calculated by comparing the measured value to the expected amount of f-Hb in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
93
EDTA plasma(n=5)
85-101
96
heparin plasma(n=5)
91-102
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of f-Hb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
f-Hb ELISA Kit allows for the in vitro quantitative determination of f-Hb concentrations in serum, plasma, tissue homogenates and other biological fluids.