Human F-actin ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13678h

Alternative Names

F-actin

Reactivity

Human

Detection Range

0.625 ng/mL-40 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse CP/CER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	92	85-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CP/CER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	95
	Range %	83-106
1:2000	Average %	92
	Range %	83-104
1:4000	Average %	95
	Range %	91-102
1:8000	Average %	93
	Range %	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.156 ng/mL

Limit Of Detection

450 nm

Antigen	F-actin

Applications

ELISA

Research Area

F-actin

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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