Human Extracellular Sulfatase Sulf-1

Mfr.No.

E5271Hu

Sensitivity

1.55ng/L

Detection Range

3-900ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Extracellular sulfatase Sulf-1;hSulf-1;SULF 1;SULF1

Molecular Weight

101027

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5271Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	108.5	4.18	3.9
Intra-Assay	2	18	184.1	6.77	3.7
Intra-Assay	3	18	348.9	11.73	3.4

Background

Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation.

Protocol

These standard curves of E5271Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	1.775	1.769	1.725
	1.763
240ng/L	1.098	1.089	1.044
	1.079
120ng/L	0.456	0.442	0.397
	0.427
60ng/L	0.243	0.222	0.177
	0.201
30ng/L	0.086	0.111	0.067
	0.136
0ng/L	0.058	0.044	0
	0.031

Gene ID	23213
UniProt ID	Q8IWU6

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106634	E5271Hu	48T	$320		Add to Cart
BHE12106634	E5271Hu	96T	$458		Add to Cart

Human Extracellular Sulfatase Sulf-1 - SULF1 ELISA Kit

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