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Home / ELISA Kit

Human Exportin-1 (XPO1) ELISA Kit

SKU : BHE13702232

Exportin-1 mediates leucine-rich nuclear export signal (NES)-dependent protein transport. Exportin 1 specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2.
This protein also regulates NFAT and AP-1.The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus.

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Mfr.No.	KTE60012
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Exportin-1 (XPO1) ELISA Kit employs a two-site sandwich ELISA to quantitate XPO1.
Alternative Names	XPO1; CRM1; DKFZp686B1823; CRM1; yeast; homolog; Exportin-1 (required for chromosome region maintenance); OTTHUMP00000201471; OTTHUMP00000201493; OTTHUMP00000201494; exportin 1; exportin 1 (CRM1; yeast; homolog
Detection Method	Colorimetric
Kit Components	Human Exportin-1 microplate Human Exportin-1 standard Human Exportin-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Exportin-1 (XPO1) ELISA Kit has high sensitivity and excellent specificity for detection of Human XPO1. No significant cross-reactivity or interference between Human XPO1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Exportin-1 mediates leucine-rich nuclear export signal (NES)-dependent protein transport. Exportin 1 specifically inhibits the nuclear export of Rev and U snRNAs. It is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. This protein also regulates NFAT and AP-1.The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus.
Notes	This Human Exportin-1 (XPO1) ELISA Kit employs a two-site sandwich ELISA to quantitate XPO1 in samples. An antibody specific for XPO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXPO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XPO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XPO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7514

Applications	ELISA
Applications Notes	This Human Exportin-1 (XPO1) ELISA Kit employs a two-site sandwich ELISA to quantitate XPO1 in samples. An antibody specific for XPO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXPO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XPO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XPO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702232	KTE60012	48 T	$339		Add to Cart
BHE13702232	KTE60012	96 T	$589		Add to Cart
BHE13702232	KTE60012	96 Tx5	$2629		Add to Cart
BHE13702232	KTE60012	96 Tx50	$24569		Add to Cart

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