Human Exostoses 1 - EXT1 ELISA Kit

Mfr.No.

E2692Hu

Sensitivity

0.024ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Exostosin-L;Exostosin-like 1;EXT1;EXTL;Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase;Multiple exostosis-like protein

Molecular Weight

74697

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2692Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.62	0.16	4.4
Intra-Assay	2	18	9.82	0.22	2.27
Intra-Assay	3	18	8.32	0.28	3.36

Background

Exostosin-1 is a protein that in humans is encoded by the EXT1 gene. This gene encodes one of the two endoplasmic reticulum-resident type II transmembrane glycosyltransferase the other being EXT2 which are involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of Multiple Exostoses.

Protocol

These standard curves of E2692Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	1.997	1.971	1.931
	1.946
8ng/ml	1.113	1.034	0.993
	0.955
4ng/ml	0.471	0.544	0.503
	0.617
2ng/ml	0.179	0.17	0.129
	0.161
1ng/ml	0.149	0.116	0.075
	0.082
0ng/ml	0.051	0.04	0
	0.03

Gene ID	2134
UniProt ID	Q92935

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104230	E2692Hu	48T	$320		Add to Cart
BHE12104230	E2692Hu	96T	$458		Add to Cart

Human Exostoses 1 - EXT1 ELISA Kit

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