Human Eukaryotic Translation Initiation Factor 4E-binding Protein 1

Mfr.No.

E5268Hu

Alternative Names

4EBP1;4E-BP1;BP-1;eIF4E-binding protein 1;EIF4EBP1;Eukaryotic translation initiation factor 4E-binding protein 1;PHAS-I;Phosphorylated heat- and acid-stable protein regulated by insulin 1

Reactivity

Human

Detection Range

20-6000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

12580

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

10.09ng/L

Background

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Gene ID	1978
UniProt ID	Q13541
Antigen	EIF4EBP1

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E5268Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.077	2.077	2.041
	2.076
1600ng/L	0.959	1.06	1.025
	1.162
800ng/L	0.615	0.558	0.523
	0.502
400ng/L	0.179	0.211	0.175
	0.244
200ng/L	0.074	0.11	0.074
	0.145
0ng/L	0.046	0.036	0
	0.026

Precision

We measured random samples of E5268Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1018.26	53.17	5.22
Intra-Assay	2	18	1474.94	50.89	3.45
Intra-Assay	3	18	1846.59	47.2	2.56

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106631	E5268Hu	48T	$320		Add to Cart
BHE12106631	E5268Hu	96T	$458		Add to Cart

Human Eukaryotic Translation Initiation Factor 4E-binding Protein 1 - EIF4EBP1 ELISA Kit

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