Human Ets Domain-containing Protein Elk-1

Mfr.No.

E5265Hu

Alternative Names

ELK 1;ELK1;ETS domain-containing protein Elk-1

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

44888

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.88ng/L

Background

Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity).

Gene ID	2002
UniProt ID	P19419
Antigen	ELK1

Research Area

Signal Transduction; Epigenetics and Nuclear Signaling

Protocol

These standard curves of E5265Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.003	1.975	1.914
	1.946
800ng/L	0.918	0.944	0.883
	0.971
400ng/L	0.541	0.527	0.466
	0.513
200ng/L	0.235	0.192	0.131
	0.149
100ng/L	0.095	0.083	0.022
	0.07
0ng/L	0.062	0.061	0
	0.059

Precision

We measured random samples of E5265Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1385	39.82	2.9
Intra-Assay	2	18	706.9	29.16	4.1
Intra-Assay	3	18	377.8	16.4	4.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106628	E5265Hu	48T	$320		Add to Cart
BHE12106628	E5265Hu	96T	$458		Add to Cart

Human Ets Domain-containing Protein Elk-1 - ELK1 ELISA Kit

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