Matrices listed below were spiked with certain level of ETNPPL and the recovery rates were calculated by comparing the measured value to the expected amount of ETNPPL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
84-99
93
EDTA plasma(n=5)
82-102
93
heparin plasma(n=5)
88-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ETNPPL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-96%
85-102%
83-104%
EDTA plasma(n=5)
90-97%
90-99%
91-100%
heparin plasma(n=5)
92-98%
98-100%
91-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q8TBG4
Applications
ELISA
Applications Notes
ETNPPL ELISA Kit allows for the in vitro quantitative determination of ETNPPL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human ETNPPL (Ethanolamine-phosphate phospho-lyase) ELISA Kit