Human Estradiol receptor,ER ELISA Kit

Mfr.No.

CSB-E08652h

Alternative Names

estrogen receptor 1, RP1-130E4.1, DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1, estrogen receptor alpha, estrogen receptor alpha delta 3*,4,5,6,7*/819-2 isoform, estrogen receptor alpha delta 4 +49 isoform, estrogen receptor

Reactivity

Human

Detection Range

3.12 pg/mL-200 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse EFNA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	80-94
EDTA plasma (n=4)	102	98-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse EFNA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	96
	Range %	93-99
1:4	Average %	87
	Range %	83-90
1:8	Average %	105
	Range %	100-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
6000	2.702	2.694	2.698	2.530
3000	2.184	2.201	2.193	2.025
1500	1.585	1.602	1.594	1.426
750	1.088	1.069	1.079	0.911
375	0.669	0.679	0.674	0.506
187.5	0.453	0.468	0.461	0.293
93.75	0.314	0.330	0.322	0.154
0	0.168	0.167	0.168	?

Species

Homo sapiens

Sensitivity

0.78 pg/mL

Limit Of Detection

450 nm

UniProt ID	P03372
Antigen	estrogen receptor 1

Applications

ELISA

Research Area

ESR1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502312	CSB-E08652h	96T	$790	Add to Cart
BHE10502312	CSB-E08652h	5x96T	$3792	Add to Cart
BHE10502312	CSB-E08652h	10x96T	$7505	Add to Cart

Human Estradiol receptor,ER ELISA Kit

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