Human Estradiol 17-beta-dehydrogenase 8

Mfr.No.

E3327Hu

Sensitivity

12.09ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

17-beta-HSD 8;17-beta-hydroxysteroid dehydrogenase 8;3-ketoacyl-(acyl-carrier-protein) reductase alpha subunit;3-oxoacyl-(acyl-carrier-protein) reductase;Estradiol 17-beta-dehydrogenase 8;HSD17B8;KAR alpha subunit;Ke-6;Protein Ke6;Really interesting new gene 2 protein;Short chain dehydrogenase/reductase family 30C member 1;Testosterone 17-beta-dehydrogenase 8

Molecular Weight

26974

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3327Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1034.4	58.37	5.6
Intra-Assay	2	18	1690.8	54.43	3.2
Intra-Assay	3	18	488.5	37.54	7.7

Background

NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone (PubMed: 17978863). The heterotetramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity, and thereby plays a role in mitochondrial fatty acid biosynthesis (PubMed: 19571038, PubMed: 25203508). Within the heterotetramer, HSD17B8 binds NADH; CBR4 binds NADPD (PubMed: 25203508).

Protocol

These standard curves of E3327Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.147	2.281	2.221
	2.414
1200ng/L	1.39	1.295	1.235
	1.2
600ng/L	0.853	0.875	0.815
	0.897
300ng/L	0.477	0.458	0.398
	0.438
150ng/L	0.324	0.296	0.236
	0.267
0ng/L	0.063	0.06	0
	0.057

Gene ID	7923
UniProt ID	Q92506

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104856	E3327Hu	48T	$320		Add to Cart
BHE12104856	E3327Hu	96T	$458		Add to Cart

Human Estradiol 17-beta-dehydrogenase 8 - HSD17B8 ELISA Kit

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