Human Erythropoietin/EPO ELISA Kit PicoKine®

Mfr.No.

EK0332

Sensitivity

<1mIU/ml

Detection Range

3.12 mIU/ml - 200 mIU/ml

Reactivity

Human

Sample Volume

Concentration (mIU/ml)	0	3.12	6.25	12.5	25	50	100	200
O.D.	0.049	0.176	0.236	0.415	0.671	1.097	1.657	2.086

Description

Human Erythropoietin/EPO ELISA Kit PicoKine® (96 Tests). Quantitate Human EPO in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 1mIU/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Erythropoietin; Epoetin; EPO

Immunogen

Expression system for standard: CHO; Immunogen sequence: A28-R193

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human EPO

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (mIU/ml)	4	23	68	5	24	76
Standard deviation	0.17	0.99	2.79	0.33	1.63	5.47
CV (%)	4.2%	4.3%	4.1%	6.6%	6.8%	7.2%

Protein Function

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

Background

Erythropoietin (EPO), also known as hematopoietin or hemopoietin, is a glycoprotein cytokine secreted by the kidney in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. It is mapped to 7q22.1. This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in tu leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.

Reproducibility

Lots	Lot 1 (mIU/ml)	Lot 2 (mIU/ml)	Lot 3 (mIU/ml)	Lot 4 (mIU/ml)	Mean (mIU/ml)	Standard Deviation	CV (%)
Sample 1	4	4	4	4	4	0.00	0.0%
Sample 2	23	23	23	21	22	0.86	3.9%
Sample 3	68	73	76	65	70	4.27	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2056
Gene Name	EPO
UniProt ID	P01588
Tissue Specificity	Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001647	EK0332	96 wells/kit	$499		Add to Cart

Human Erythropoietin/EPO ELISA Kit PicoKine®

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