Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of ERCC1 and the recovery rates were calculated by comparing the measured value to the expected amount of ERCC1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
94
EDTA plasma(n=5)
87-103
94
heparin plasma(n=5)
85-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ERCC1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ERCC1 ELISA Kit allows for the in vitro quantitative determination of ERCC1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human ERCC1 (DNA excision repair protein ERCC-1) ELISA Kit