Matrices listed below were spiked with certain level of ERAP2 and the recovery rates were calculated by comparing the measured value to the expected amount of ERAP2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-95
91
EDTA plasma(n=5)
85-97
91
heparin plasma(n=5)
85-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ERAP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-102%
87-105%
89-104%
EDTA plasma(n=5)
87-101%
84-94%
89-97%
heparin plasma(n=5)
83-97%
85-91%
83-96%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q6P179
Antigen
ERAP2
Applications
ELISA
Applications Notes
ERAP2 ELISA Kit allows for the in vitro quantitative determination of ERAP2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human ERAP2(Endoplasmic reticulum aminopeptidase 2) ELISA Kit