Human Epsin 1 - EPN1 ELISA Kit

Mfr.No.

E2678Hu

Sensitivity

10.25ng/L

Detection Range

20-6000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

EH domain-binding mitotic phosphoprotein;EPN 1;EPN1;EPS-15-interacting protein 1;Epsin-1

Molecular Weight

60293

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2678Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1416.7	40.32	2.8
Intra-Assay	2	18	326.6	15.26	4.7
Intra-Assay	3	18	1168.6	58.07	5

Background

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.

Protocol

These standard curves of E2678Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.382	2.405	2.358
	2.428
1600ng/L	1.404	1.377	1.331
	1.35
800ng/L	0.891	0.887	0.841
	0.884
400ng/L	0.401	0.415	0.368
	0.429
200ng/L	0.218	0.212	0.165
	0.205
0ng/L	0.052	0.046	0
	0.04

Gene ID	29924
UniProt ID	Q9Y6I3

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104216	E2678Hu	48T	$320		Add to Cart
BHE12104216	E2678Hu	96T	$458		Add to Cart

Human Epsin 1 - EPN1 ELISA Kit

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