Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) ELISA Kit

Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) ELISA Kit

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Mfr.No.

CSB-E08178h

Sensitivity

0.567 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-X-C motif) ligand 5, ENA-78, SCYB5, epithelial-derived neutrophil activating protein 78, epithelial-derived neutrophil-activating peptide 78, neutrophil-activating peptide ENA-78, neutrophil-activating protein 78, small ind

Recovery

The recovery of horse IGF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-101
EDTA plasma (n=4)	87	82-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse IGF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-95
1:2	Average %	95
	Range %	90-104
1:4	Average %	92
	Range %	87-100
1:8	Average %	89
	Range %	80-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
800	1.961	1.944	1.953	1.820
400	1.488	1.605	1.547	1.414
200	1.178	1.213	1.196	1.063
100	0.912	0.902	0.907	0.774
50	0.669	0.657	0.663	0.530
25	0.405	0.396	0.401	0.268
12.5	0.260	0.249	0.255	0.122
0	0.137	0.129	0.133

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P42830

Applications	ELISA
Research Area	CXCL5

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502293	CSB-E08178h	96T	$375	Add to Cart
BHE10502293	CSB-E08178h	5x96T	$1800	Add to Cart
BHE10502293	CSB-E08178h	10x96T	$3562.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.