Human Epithelial Cell Adhesion Molecule

Mfr.No.

E0307Hu

Sensitivity

0.096ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Epithelial Cell Adhesion Molecule (also known as Ep-CAM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Adenocarcinoma-associated antigen;CD antigen CD326;Cell surface glycoprotein Trop-1;DIAR5;EGP;EGP-2;EGP314;Ep-CAM;Epithelial cell adhesion molecule;Epithelial cell surface antigen;Epithelial glycoprotein;Epithelial glycoprotein 314;ESA;hEGP314;HNPCC8;KS 1/4 antigen;KS1/4;KSA;M4S1;Major gastrointestinal tumor-associated protein GA733-2;MIC18;MK-1;TACSTD1;TROP1;Tumor-associated calcium signal transducer 1

Molecular Weight

34932

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0307Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	20.02	0.64	3.21
Intra-Assay	2	18	11.72	0.7	5.97
Intra-Assay	3	18	7.11	0.52	7.36

Background

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Protocol

These standard curves of E0307Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.098	2.171	2.139
	2.244
16ng/ml	1.312	1.26	1.228
	1.209
8ng/ml	0.634	0.671	0.639
	0.707
4ng/ml	0.501	0.514	0.482
	0.526
2ng/ml	0.18	0.215	0.183
	0.25
0ng/ml	0.033	0.032	0
	0.031

Gene ID	4072
UniProt ID	P16422

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102012	E0307Hu	48T	$320		Add to Cart
BHE12102012	E0307Hu	96T	$458		Add to Cart

Human Epithelial Cell Adhesion Molecule - EP-CAM ELISA Kit

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