Human Epinephrine/Adrenaline,EPI ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08677h

Sensitivity

1 pg/mL

Detection Range

2 pg/mL-400 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Epinephrine/Adrenaline,EPI

Recovery

The recovery of human EFEMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-94
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human EFEMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	98-109
1:2	Average %	84
	Range %	80-88
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	EPI

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Acute exercise increases fibroblast growth factor 21 in metabolic organs and circulation. Tanimura Y.et al,Physiol Rep.,4(12): e12828. Effect of local wound infiltration with ropivacaine on postoperative pain relief and stress response reduction after open hepatectomy Jing-Xian Sun.et al,World J Gastroenterol,Sep 28

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