Human epididymal protein 4(HE4) ELISA kit

Human epididymal protein 4(HE4) ELISA kit

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Mfr.No.

CSB-E12923h

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Sample Type

Serum

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

WAP four-disulfide core domain 2, EDDM4, HE4, MGC57529, WAP5, dJ461P17.6, WAP domain containing protein HE4-V4, epididymal protein 4, epididymal secretory protein E4, epididymis-specific, whey-acidic protein type, four-disulfide core, m

Recovery

The recovery of rat BK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	93-96
EDTA plasma (n=4)	100	95-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat BK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	88
	Range %	84-92
1:10	Average %	94
	Range %	90-98
1:20	Average %	99
	Range %	95-104
1:40	Average %	102
	Range %	96-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
50	2.640	2.698	2.669	2.497
25	1.835	1.846	1.841	1.669
12.5	1.112	1.136	1.124	0.952
6.25	0.729	0.710	0.720	0.548
3.12	0.498	0.475	0.487	0.315
1.56	0.335	0.316	0.326	0.154
0.78	0.251	0.259	0.255	0.083
0	0.171	0.173	0.172	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q14508
Antigen	WAP four-disulfide core domain 2

Applications	ELISA
Research Area	WFDC2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502278	CSB-E12923h	96T	$835	Add to Cart
BHE10502278	CSB-E12923h	5x96T	$4008	Add to Cart
BHE10502278	CSB-E12923h	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.