Human epidermal growth factor receptor 2 (sp185/HER2) ELISA Kit

Human epidermal growth factor receptor 2 (sp185/HER2) ELISA Kit

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Mfr.No.

CSB-E11161h

Sensitivity

0.04 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), CD340, HER-2, HER-2/neu, HER2, NEU, NGL, TKR1, c-erb B2/neu protein, erbB-2, herstatin, neuroblastoma/glioblastoma derived oncogene homolog, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog

Recovery

The recovery of mouse NLRP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	100	96-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NLRP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	96
	Range %	92-101
1:4	Average %	88
	Range %	85-93
1:8	Average %	89
	Range %	83-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.217	2.406	2.312	2.138
200	1.241	1.302	1.272	1.098
100	0.737	0.762	0.750	0.576
50	0.449	0.478	0.464	0.290
25	0.373	0.347	0.360	0.186
12.5	0.297	0.280	0.289	0.115
6.25	0.205	0.203	0.204	0.030
0	0.170	0.178	0.174

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P04626

Applications	ELISA
Research Area	ERBB2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502276	CSB-E11161h	96T	$695	Add to Cart
BHE10502276	CSB-E11161h	5x96T	$3336	Add to Cart
BHE10502276	CSB-E11161h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.