Human Ephrin-B2(EFNB2) ELISA kit

Human Ephrin-B2(EFNB2) ELISA kit

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Mfr.No.

CSB-EL007466HU

Alternative Names

ephrin-B2, RP11-272L14.1, EPLG5, HTKL, Htk-L, LERK5, MGC126226, MGC126227, MGC126228, HTK ligand, eph-related receptor tyrosine kinase ligand 5, ephrin B2, ligand of eph-related kinase 5

Reactivity

Human

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of fish GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	94	89-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	93-104
1:2	Average %	94
	Range %	85-103
1:4	Average %	95
	Range %	83-102
1:8	Average %	92
	Range %	87-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
20	0.134	0.140	0.137
10	0.232	0.242	0.237
4.6	0.398	0.409	0.404
1.5	0.714	0.687	0.701
0.5	1.293	1.266	1.280
0	2.711	2.740	2.726

Species

Homo sapiens

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	P52799
Antigen	Ephrin-B2

Applications

ELISA

Research Area

EFNB2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502255	CSB-EL007466HU	96T	$695	Add to Cart
BHE10502255	CSB-EL007466HU	5x96T	$3336	Add to Cart
BHE10502255	CSB-EL007466HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.