Human Ephrin-A1(EFNA1) ELISA kit

Mfr.No.

CSB-EL007460HU

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ephrin-A1, B61, ECKLG, EFL1, EPLG1, LERK1, TNFAIP4, eph-related receptor tyrosine kinase ligand 1, ephrin A1, immediate early response protein B61, ligand of eph-related kinase 1, tumor necrosis factor, alpha-indu

Recovery

The recovery of NDM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-96
EDTA plasma (n=4)	96	87-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of NDM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-103
1:2	Average %	92
	Range %	82-99
1:4	Average %	93
	Range %	87-97
1:8	Average %	95
	Range %	89-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	1.845	1.838	1.842	1.698
200	1.606	1.619	1.613	1.469
100	1.337	1.340	1.339	1.195
50	0.983	0.972	0.978	0.834
25	0.606	0.598	0.602	0.458
12.5	0.379	0.370	0.375	0.231
6.25	0.250	0.247	0.249	0.105
0	0.145	0.143	0.144	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P20827

Applications	ELISA
Research Area	EFNA1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502254	CSB-EL007460HU	96T	$695	Add to Cart
BHE10502254	CSB-EL007460HU	5x96T	$3336	Add to Cart
BHE10502254	CSB-EL007460HU	10x96T	$6602.50	Add to Cart

Human Ephrin-A1(EFNA1) ELISA kit

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