Human Enteropeptidase(PRSS7) ELISA kit

Mfr.No.

CSB-EL018824HU

Alternative Names

protease, serine, 7 (enterokinase), ENTK, MGC133046, enterokinase, enteropeptidase

Reactivity

Human

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-90
EDTA plasma (n=4)	100	95-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-88
1:2	Average %	92
	Range %	89-96
1:4	Average %	102
	Range %	98-105
1:8	Average %	99
	Range %	95-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	P98073
Antigen	protease, serine, 7 (enterokinase)

Applications

ELISA

Research Area

PRSS7

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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