Human ENPP7 ELISA Kit PicoKine®

Mfr.No.

EK2191

Sensitivity

<10 pg/ml

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	93.7	187.5	375	750	1,500	3,000	6,000
O.D.	0.048	0.096	0.212	0.481	0.762	1.021	1.528	2.032

Description

Human ENPP7 ELISA Kit PicoKine® (96 Tests). Quantitate Human ENPP7 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A22-S439

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Human ENPP7

Sample Type

Cell culture supeatants, Serum, Plasma

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	100	329	842	116	423	915
Standard deviation	5.5	17.44	51.36	5.22	20.73	51.24
CV(%)	5.5%	5.3%	6.1%	4.5%	4.9%	5.6%

Background

Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (E-NPP 7) also known as alkaline sphingomyelin phosphodiesterase (Alk-SMase) or intestinal alkaline sphingomyelinase is an enzyme that in humans is encoded by the ENPP7 gene. The protein encoded by this gene is an intestinal alkaline sphingomyelin phosphodiesterase that converts sphingomyelin to ceramide and phosphocholine. The encoded protein is anchored in the cell membrane, and it may function to protect the intestinal mucosa from inflammation and tumorigenesis. This protein is glycosylated and also exhibits lysophosphatidylcholine hydrolase activity.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	100	118	107	95	105	5.57	5.3%
Sample 2	329	341	336	329	334	17.02	5.1%
Sample 3	842	856	844	837	845	49	5.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	339221
Gene Name	ENPP7
UniProt ID	Q6UWV6

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001736	EK2191	96 wells/kit	$499		Add to Cart

Human ENPP7 ELISA Kit PicoKine®

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