Human endothelial cell-specific molecule 1(ESM-1) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E16530h

Reactivity

Human

Sample Type

Cell Lysates, Serum, Tissue Homogenates, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rabbit MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	93	89-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-103
1:2	Average %	87
	Range %	84-90
1:4	Average %	95
	Range %	89-99
1:8	Average %	101
	Range %	98-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

UniProt ID	Q9NQ30
Antigen	endothelial cell-specific molecule 1

Applications

ELISA

Research Area

ESM1

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10502237	CSB-E16530h	96T	$695	Add to Cart
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BHE10502237	CSB-E16530h	10x96T	$6602.50	Add to Cart