Human Endosialin(CD248) ELISA kit

Human Endosialin(CD248) ELISA kit

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Mfr.No.

CSB-EL004905HU

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

CD248 molecule, endosialin, CD164L1, MGC119478, MGC119479, TEM1, 2610111G01Rik, CD164 sialomucin-like 1, CD248 antigen, endosialin, endosialin, tumor endothelial marker 1

Recovery

The recovery of mouse PARK7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	92	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PARK7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-102
1:2	Average %	96
	Range %	91-101
1:4	Average %	95
	Range %	91-99
1:8	Average %	96
	Range %	92-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	3.057	3.104	3.081	2.911
2000	2.577	2.472	2.525	2.355
1000	1.669	1.683	1.676	1.506
500	0.942	1.000	0.971	0.801
250	0.555	0.575	0.565	0.395
125	0.390	0.415	0.403	0.233
62.5	0.283	0.302	0.293	0.123
0	0.169	0.171	0.170	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9HCU0

Applications	ELISA
Research Area	CD248

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502233	CSB-EL004905HU	96T	$695	Add to Cart
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BHE10502233	CSB-EL004905HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.