Human Endorepellin/HSPG2 ELISA Kit PicoKine®

Mfr.No.

EK1760

Sensitivity

<0.05 ng/ml

Detection Range

0.624 ng/ml - 40 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.62	1.25	2.5	5	10	20	40
O.D.	0.016	0.088	0.195	0.463	0.737	0.829	1.631	1.893

Description

Human Endorepellin/HSPG2 ELISA Kit PicoKine® (96 Tests). Quantitate Human HSPG2 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 0.05ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Basement membrane-specific heparan sulfate proteoglycan core protein; HSPG; Perlecan; PLC; HSPG2

Immunogen

Expression system for standard: NS0; Immunogen sequence: E3687-S4391

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Endorepellin/HSPG2

Subcellular Localization

basement membrane

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1098	3228	15773	1118	4325	18932
Standard deviation	58.19	132.3	1056.8	60.4	311.4	1438.83
CV (%)	5.3%	4.1%	6.7%	5.4%	7.2%	7.6%

Protein Function

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachm

Background

Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1098	1094	1079	1015	1071	33.38	3.1%
Sample 2	3228	3312	3233	3315	3272	41.55	1.2%
Sample 3	15773	17166	16982	16097	16504	584.53	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3339
Gene Name	HSPG2
UniProt ID	P98160
Tissue Specificity	Found in the basement membranes.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cancer Metabolism,Cardiovascular,Cytoskeleton/ECM,Extracellular Matrix,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Pathways and Processes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001387	EK1760	96 wells/kit	$750		Add to Cart

Human Endorepellin/HSPG2 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Endorepellin/HSPG2 ELISA Kit PicoKine®

Rate it now..!!