Human Endonuclease G - Mitochondrial

Mfr.No.

E5235Hu

Sensitivity

10.12ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Endo G;ENDOG;Endonuclease G, mitochondrial

Molecular Weight

32620

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5235Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1056.55	59.09	5.59
Intra-Assay	2	18	1536.67	44.73	2.91
Intra-Assay	3	18	494.02	30.36	6.15

Background

Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (By similarity).

Protocol

These standard curves of E5235Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.418	2.295	2.249
	2.171
1200ng/L	1.394	1.31	1.264
	1.226
600ng/L	0.761	0.795	0.749
	0.828
300ng/L	0.451	0.444	0.398
	0.437
150ng/L	0.237	0.239	0.193
	0.241
0ng/L	0.043	0.046	0
	0.048

Gene ID	2021
UniProt ID	Q14249

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106598	E5235Hu	48T	$320		Add to Cart
BHE12106598	E5235Hu	96T	$458		Add to Cart

Human Endonuclease G - Mitochondrial - ENDOG ELISA Kit

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