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Home / ELISA Kit

Human Endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit

SKU : BHE13703345

Endocrine gland vascular endothelial growth factor encoded by EG-VEGF induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner.

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Mfr.No.	KTE61129
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit employs a two-site sandwich ELISA to quantitate PROK1.
Alternative Names	PROK1; EGVEGF; PK1; PRK1; black mamba toxin-related protein; mambakine
Detection Method	Colorimetric
Kit Components	Human Endocrine gland vascular endothelial growth factor microplate Human Endocrine gland vascular endothelial growth factor standard Human Endocrine gland vascular endothelial growth factor detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit has high sensitivity and excellent specificity for detection of Human PROK1. No significant cross-reactivity or interference between Human PROK1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Endocrine gland vascular endothelial growth factor encoded by EG-VEGF induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner.
Notes	This Human Endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit employs a two-site sandwich ELISA to quantitate PROK1 in samples. An antibody specific for PROK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	84432

Applications	ELISA
Applications Notes	This Human Endocrine gland vascular endothelial growth factor (EG-VEGF) ELISA Kit employs a two-site sandwich ELISA to quantitate PROK1 in samples. An antibody specific for PROK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703345	KTE61129	48 T	$399		Add to Cart
BHE13703345	KTE61129	96 T	$739		Add to Cart
BHE13703345	KTE61129	96 Tx5	$3339		Add to Cart
BHE13703345	KTE61129	96 Tx50	$29639		Add to Cart

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