Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit

Mfr.No.

FEK0751

Sensitivity

<2 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1,000	2,000
O.D.	0.029	0.097	0.155	0.262	0.484	0.879	1.548	2.261

Description

Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human EMMPRIN/CD147/BSG in cell culture supeatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T25-H205

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Human BSG

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Urine, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	71	234	1067	72	212	977
Standard deviation	4.68	16.84	84.29	5.40	15.90	79.13
CV(%)	6.6%	7.2%	7.9%	7.5%	7.7%	8.1%

Background

Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147(cluster of differentiation 147). It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition(Miyauchi et al., 1991; Kanekura et al., 1991).This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin(CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	71	71	66	67	68	2.58	3.8%
Sample 2	234	209	223	212	219	11.38	5.2%
Sample 3	1067	994	957	1024	1010	45.45	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	682
Gene Name	BSG
UniProt ID	P35613

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000271	FEK0751	96 wells/kit	$499		Add to Cart

Human EMMPRIN/CD147/BSG PicoKine® Quick ELISA Kit

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Reproducibility

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